Fig 1: The expression of PD-L1 in kidney tissue of mice. The IHC of PD-L1 was shown in control groups (A), sham groups (B) and IRI groups (C). Quantification is represented in panel (D). Data are expressed as mean ± standard deviation. Difference between IRI group and sham group was made using unpaired Student’s t-test for normally distributed data. Images, × 200; original scale bar, 100 µm. **p<0.01. ***p<0.001. Images, × 200; original scale bar, 100 µm.
Fig 2: PCR results for PD-1, PD-L1, CTLA-4, IFN-?, IL-6 and Survivin mRNA in various tissues. CT = control, siPD-1 = group injected with siRNA for PD-1, siCTLA-4 = group injected with siRNA for CTLA-4, siPD-1 + siCTLA-4 = group injected with siRNA for PD-1 + CTLA-4. a – Level of IFN-? mRNA in the tumors. b – Level of Survivin mRNA in the tumors. c – Level of PD-L1 mRNA in the tumors. d – Level of IL-6 mRNA in the tumors. e – Level of PD-1 mRNA in the spleen. f – Level of CTLA-4 mRNA in the spleen. g – Level of IFN-? mRNA in the spleen. h – Level of IFN-? mRNA in the liver. i – Level of PD-L1 mRNA in the liver. The mRNA expressions of some genes in the siRNA groups were significantly different than those in the control group (*p < 0.05, **p < 0.01)
Fig 3: The concentrations of sPD-1 and sPD-L1 in plasma and the concentrations of PD-1 and PD-L1 in kidney homogenate (A). Correlation was shown between sPD-1 in plasma and PD-1 in kidney homogenate (r = 0.774, p<0.001) (B), between sPD-L1 in plasma and PD-L1 in kidney homogenate (r = 0.881 p<0.001) (B) using Spearman’s test.
Fig 4: IFN-?, IL-10 and PD-L1 levels in the blood and tissue. These results are based on ELISA tests. CT = control, siPD-1 = group injected with siRNA for PD-1, siCTLA-4 = group injected with siRNA for CTLA-4, siPD-1 + siCTLA-4 = group injected with siRNA for PD-1 + CTLA-4. a – IFN-?level in mouse serum. b – IL-10 level in mouse serum. c – PD-L1 level in tumor tissue. d – PD-L1 content in liver tissue. The expressions of IFN-?, IL-10 and PD-L1 in the blood and tissue of the siRNA groups were significantly different from those in the control group (**p < 0.01, *p < 0.05)
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